Isolation and Studying Cathepsin B Enzyme from Normal Human Serum

Comparative studies of two cathepsin B isozymes from porcine spleen. Isolation, polypeptide chain arrangements, and enzyme specificity.

Our previous studies on carbohydrate structures of purified porcine spleen cathepsin B indicated that there are two cathepsin B isozymes, each containing a different carbohydrate (Takahashi, T., Schmidt, P.G., and Tang, J. (1984) J. Biol. Chem. 259, 6059-6062). We have now isolated these two enzymes and carried out a comparative study on their structures and enzymic properties. The major isozym...

THE ISOLATION OF ENZYME TRANSKETOLASE FROM HUMAN ERYTHROCYTES: THE CHARACTERIZATION OF ITS QUARTERNARY STRUCTURE

Human erythrocyte transketolase (sedoheptulose-7-phosphate: D-glyceraldehyde-3-phosphate, glycolaldehyde transferase, E.C. 2.2.1.1.) has been isolated from erythrocytes with a specific activity of 59.84 U/mg. SDS-PAGE and SE-HPLC were used both as a measure of purity and as a preparative mean to obtain a higher degree of purity. Four protein bands corresponding to molecular weights of 32,0...

Isolation and properties of lipoproteins from normal rat serum.

Three major classes of lipoproteins (VLDL, d <1.006; LDL, d 1.006-1.040; HDL, d 1.063-1.21) were isolated by ultracentrifugal flotation from the serum of normal male Sprague-Dawley rats. Their physical, chemical, and immunological properties were analyzed and compared with those of their water-soluble, essentially lipid-free derivatives. Studies were also carried out on the d > 1.21 fractions. ...

Isolation and identification of lysolecithin from lipid extracts of normal human serum.

Isolation of elastase from human serum.

The mean increase in activation of the MgZ+-ATPase was 2.95f1.23 (n = 5 ) for muscle myosin and 2.90 for the platelet myosin and the most favourable combining ratios (w/w) for both lay between 2:l and 4:l actin to myosin. An antibody has been raised to the leucocyte actin, and we have used this for the detection of actin filaments in a variety of cells, including guinea-pig polymorphonuclear le...